The aim of this study was to develop the immunohistochemical method for detection of the Bovine Spongiform Encephalopathy (BSE) specific protein (PrP) in bovine brain tissue that is free from cross reactions. A slaughterhouse surveillance was conducted in two districts (Dhaka and Mymensingh). Around 1000 brain samples were collected from 30 months old cows, slaughtered for human consumption. The brainstem (obex), pyriform lobe, pieces of cerebellum and cerebrum were used to detect BSE specific protein, using an anti PrPsc monoclonal antibody (prionic AG, Switzerland). A labelled Streptavidin Biotin (LSAB) method of immunohistochemical staining for the detection of BSE specific protein (PrPn in paraffin embedded positive tissue sections was developed successfully. PrPsc was detected in the cytoplasm of neurons of positive brain tissues. Some of the brain tissues collected from field showed some gliosis and inflammatory lesions but none of• the lesions were consistent with BSE histopathologically and all of the samples were negative for the PrPsc protein by immunohistochemical method. The developed protocol may be used as tool for the diagnosis of BSE.