An experiment was conducted at the Agronomy Field Laboratory of Bangladesh Agricultural University (BAU), Mymensingh during February to May 2005 with a view to studying the effect of variety and sulphur on the seed yield, oil content and protein content of sesame. The experiment consisted of three sesame varieties (BINA Ti1-1, BARI Ti1-2 and Local) and four sulphur levels (0, 9, 18 and 27 kg S ha-1). The experiment was laid out in a randomized complete block design with three replications. The result showed significant variations among the varieties for all the studied crop characters. The highest seed yield, stover yield, oil content and harvest index was recoded with BINA Ti1-1 and the lowest performance was with local variety. Sulphur fertilization showed significant influence on the studied plant characters except length of capsule, 1000-seed weight and stover yield. The highest seed yield, oil content and protein content were achieved from the application of 27 kg S ha -I which was not significantly different from that of 18 kg S ha.1. On overall consideration, the best performance with respect to seed yield, oil and protein content was obtained from the combination of BINA Ti1-1 and application of 18 kg S ha-1.