With a view to develop and establish a reproducible protocol for rapid regeneration in tomato, leaf and internodes explants of tamato were used. Leaf and internodes explants were cultured on MS medium containing 30 0-1 sucrose, 8 g1-1 agar supplemented with a-Naphthalen acetic acid (NAA), Indole acetic acid (IAA) and 6-Benzyl amino purine (BAP). Explants were sterilized and cultured on MS medium supplemented with BAP at 2 to 3 mg1-1 in combination with NAA at 0.1, 0.25 and 0.5 mg' for callus induction. The combination of BAP at 3mg1-1 and NAA at 0.25 mg1-1 was the most suitable treatment for callus formation. Internode explants produced higher amount of callus than the other explants. Leaf explants produced highest number of shoot at 1.0 mgli BAP. Regenerated shoots were excised and then transferred to medium containing various levels of IAA for rooting. IAA at 0.25 mg1-1 produced large number of roots. Internode explants were more suitable for root production.