The growth performance of Spirulina platensis was studied in various concentrations viz., 0.3, 0.4 and 0.5 g/L of papaya skin powder medium (PSPM) and Kosaric Medium (KM) in the laboratory for three months. Each trial was carried out for a period of 12 days with three replications. The growth rate of S. platensis was found to vary in different media. The initial cell weight of S. platensis was 4.0 mg/L which attained a maximum weight of 0.720 g/L in KM followed by 0.693, 0.578 and 0.430 g/L in 0.3, 0.4 and 0.5 g/L of PSPM, respectively on 8th day of culture. Similar trend was observed in the case of chlorophyll a content of S. platensis. The proximate composition of papaya skin powder was analyzed and found that the amount of protein was 20.25%, lipid 3.85%, ash 9.31%, NFE 50.49% and moisture 16.10%. The result indicated that the growth rate of S. platensis was significantly (p<0.01) higher in 0.3 g/L concentration of PSPM than other concentrations of PSPM. The physico-chemical parameters viz., temperature (30.06 °C), light intensity (2110 lux/m2/s), dissolved oxygen (4.8 mg/L), pH (12.08), nitratenitrogen (3.29 mg/L), phosphate-phosphorus (1.97 mg/L), and nitrite-nitrogen (0.063 mg/L) were observed in optimum levels.