Immature zygotic embryos of Piper nigrum L. were cultured in different concentrations and combination of auxins and cytokinins. Callus formation was highest (92.2%) when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/I IAA and 0.5 mg/I kinetin. CaIli were transferred to same basal medium containing 1.5 mg/I BAP and 0.5 mg/I NAA, where they developed maximum number of adventitious buds within three weeks of culture. Regenerated shoots were elongated on MS medium containing 1.5 mg/I BAP and 0.5 mg/I NAA and subsequently rooted in halfstrength MS medium with 1.5 mg/I IBA.