In vitro callus induction and plant regeneration from immature zygotic embryos of black pepper (Piper nigrum L.)

Immature zygotic embryos of Piper nigrum L. were cultured in different concentrations and combination of auxins and cytokinins. Callus formation was highest (92.2%) when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/I IAA and 0.5 mg/I kinetin. CaIli were transferred to same basal medium containing 1.5 mg/I BAP and 0.5 mg/I NAA, where they developed maximum number of adventitious buds within three weeks of culture. Regenerated shoots were elongated on MS medium containing 1.5 mg/I BAP and 0.5 mg/I NAA and subsequently rooted in halfstrength MS medium with 1.5 mg/I IBA.