Garlic bulbs (A/hum sativum) extracts were made in benzene, diethyl ether, dichloromethane and water. at room temperature (25±2 °C). The solvents were These extracts were applied to determine their inhibitory effects against Phomopsis vexans. TLC of these extracts was done by using different pure and suitably mixed solvents.Growth inhibition test of Phomopsis vexans by each of these extracts in different solvents revealed different mode of action. Dichloromethane extract of garlic were found to be very satisfactory in inhibition (83.52-86.45 %).TLC of these extracts showed the presence of a number of compounds having very low to high polarity. Four single compounds were separated and tested against Phomopsis vexans individually. Among the separated compounds, three compounds P1B1, BlEi and E were completely inhibitory against Phomopsis vexans while the other compound P1133 had little inhibitory effect. The Rf values of TLC spots produced by different compounds present in garlic extract revealed their distinct polar and non polar nature. These studies lead us in near future to the elucidation of complete structure of these active compounds.