The research was carried out to compare the physical and chemical parameters of swamp and water buffalo milk collected from Senbari village in Trishal upazila under Mymensingh district. Results revealed that average colour, flavour and taste score of swamp and water buffalo milk differed (p<0.01) and the mean values were 91.10 ± 0.95 vs 87.50 ± 0.83, 96.30 ± 0.64 vs 86.10 ± 0.95 and 95.00 ± 0.00 vs 90.37 ± 2.32 for swamp and water buffalo respectively. The specific gravity of swamp buffalo milk (1.032 ± 0.00) slightly differed (p>0.05) with water buffalo milk (1.030 ± 0.00). Texture of all m;lk samples was normal. From chemical analyses it was observed that average fat, total solids (TS), solidsnot- fat (SNF) and protein content differed (p<0.01) and the mean values were 84.25 ± 0.25 vs 72.67 ± 0.58, 179.05 ± 0.35 vs 164.87 ± 0.02, 94.80 ± 0.15 vs 92.20 ± 0.1939 and 39.68 ± 0.06 vs 37.67 ± 0.26 g/kg for swamp and water buffalo respectively. However, no differences (p>0.05) were found among ash (7.13 ± 0.00 Vs 7.07 ± 0.02), acidity (0.164 ± 0.00 vs 0.155 ± 0.00), water (826.600 ± 5.55 vs 832.967 ± 0.06) and pH (6.52 ± 0.124 vs 6.37 ± 0.017) content of milk obtained from swamp and water buffalo. From these results it may me inferred that the quality of swamp buffalo milk is superior to water buffalo milk.