An experiment was conducted to characterize 62 mango germplasm using peroxidase isoenzyme. The results of the experiment revealed a wide range of diversity among the germplasm based on their peroxidase isoenzyme banding patterns. In respect of isoenzyme activity, nine bands were found at different Rf values located in three loci. Band number one and two were common in all the germplasm. Third locus is the most useful isoenzyme locus, which denotes allelic ipolymorphism in the germplasm MI 11 MI 48 MI 52 MI 69 but other loci do not denote allelic polymorphism.