Different combinations of BAP, IAA, NAA, Kn and Ads were used to observe the regeneration capacity of groundnut from calli in MS medium. Four groundnut genotypes were included in the study. The responses of shoot differentiation in different treatments were different. The medium supplemented with 1.0 mg/I BAP + 1.0 mg/I IAA showed the highest percentage of shoot regeneration (40 to 75%) while the lowest (0 to 60%) was observed in MS + 1.0 mg/I BAP + 2.0 mg/I NAA + 75.0 mg/I Ads. Half strength basal MS (1/2 MS) medium 'with IBA was used to investigate the rooting response of regenerated shoots. The highest percentage of root induction was observed by the genotypes Dhaka-1 and DG-2 in 0.5 mg/I IBA and the lowest by Acc.-12 in both the concentrations of IBA. Small plantlets with well develop root system were removed from the medium and transplanted in small pots. The pots were transferred to polythene shed for hardening. Survival rate of plantlets were about 75% for all the . genotypes under study.