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Abstract
An experiment was conducted to determine the comparative efficacy of experimentally prepared formalin killed (FK) and heat killed (HK) fowl cholera (FC) vaccines with virulent avian Pasteurella multocida (PM 38) serotype 1 (X-73). The virulence of vaccine strain of P. multocida was studied in mice and chickens. The comparative efficacy of experimentally prepared Formalin killed fowl cholera (FK FC) vaccine and Heat killed fowl cholera (HK FC) vaccine was determined by vaccinating 2 months old 20 chicks and each group consisted of 5 birds. Birds of group A and group B were immunized with FK FC vaccine by intramuscular (IM) and subcutaneous (SC) route respectively and that of group C and group D were immunized with HK FC vaccine by IM and SC route respectively. All the chickens were immunized twice at 15 days interval and serum was collected from every bird after each vaccination and challenge experiment. The presence of antibody against P. multocida was determined by serum agglutination test (SAT) and growth inhibition test (GIT). The post vaccination serum titres were determined by passive hemagglutination test (PHA). It was demonstrated that experimental FK FC vaccine conferred 100% protection against challenge infection when HK FC vaccine protected 80% vaccinated birds.