An experiment was conducted in different farms of BINA sub-station at Magura and Ishurdi and in the farmers’ field at Nachole upazila of Chapai Nowabgonj and Tetulia upazila of Panchagor during kharif-I to evaluate the performance of sesame mutant lines (SM-5 and SM-12). The tested parameters included: plant height, branches per plant, capsules per plant, seeds per capsule, days to maturity and yield per ha. Highly significant variations were observed both in individual location and combined over locations for all traits except number of capsules per plant in on-station trial. On the other hand, highly significant variations were observed for all traits except capsules per plant in Nachole upazila of Chapai Nowabgonj and combined over locations. The mutant line SM-12 can be registered as a new variety of sesame in respect of higher number of branches per plant, capsules per plant and higher seed yield.