Taking the case of vocational schools in Xuzhou City, we analyze the challenges and opportunities faced by the connotation construction of agriculture-related majors in vocational schools. And we discuss the focus of connotation construction of agriculture-related majors, by establishing one theme, building two aspects, deepening three reforms and highlighting four tasks, aiming to make the connotation construction of agriculture-related majors get somewhere, and provide strong support for agricultural science and technology innovation. Finally we put forth measures for the connotation construction of agriculture-related majors.