Based on the perception of knowledge management from experts specification in different fields, and experts at home and abroad, the knowledge management of agricultural scientific research institution can build new platform, offer new approach for realization of explicit or tacit knowledge, and promote resilience and innovative ability of scientific research institution. The thesis has introduced functions of knowledge management research of agricultural science. First, it can transform the tacit knowledge into explicit knowledge. Second, it can make all the scientific personnel share knowledge. Third, it is beneficial to the development of prototype system of knowledge management. Fourth, it mainly researches the realization of knowledge management system. Fifth, it can manage the external knowledge via competitive intelligence. Sixth, it can manage foster talents of knowledge management for agricultural scientific institution. Seventh, it offers the decision-making service for leaders to manage scientific program. The thesis also discusses the content of knowledge management of agricultural scientific research institution as follows: production and innovation of knowledge; attainment and organizing of knowledge; dissemination and share of knowledge; management of human resources and the construction and management of infrastructure. We have put forward corresponding countermeasures to further reinforce the knowledge management research of agricultural scientific research institution.