An economic analysis of tissue-cultured banana (TCB) and sucker-propagated banana (SPB) has been presented through studying their costs and returns. The factors influencing the costs of their production have been identified and resource-use efficiency has been studied. The risks in cultivation of tissue-cultured banana have been highlighted. The study has been conducted in the Theni district of Tamil Nadu using personal interview method. Probit model has been employed to find out the factor influencing the adoption of tissue culture. The study has revealed that tissue-cultured banana is more profitable to farmers than sucker-propagated banana. The resources could be utilized efficiently in TCB. Gross income and bunch weight are the major factors influencing the adoption of TCB. Also, the risk is lower in TCB than in SPB. The study has suggested that farmers should be encouraged to adopt TCB to get higher yield and profits.