The research investigated gender participation in cassava processing activities in Ayetoro area of Ogun State. Cassava is a staple food in Nigeria generally processed into “gari”, “lafun” and “fufu”. Male and female processors participate in the various processing activities with the use of different processing techniques. Purposive and random sampling techniques were adopted for the study. Four villages were chosen and 240 respondents comprising of male and female processors in “gari”, “lafun” and “fufu” processing. The data were analyzed using both percentage distribution and analysis of variance. The study reveals that 37.5% of male processors were between 41-50 years while 30.6% of female processor was between 31 – 40 years. Majority (60.4%) of female processors were Christians while (50.0%) of the male processors were Muslim. The household size of most of male processors (50.0%) ranges between 6-10 members while that of 56.9% of female processors range between 1 -5 members. The study further shows that 40.0% of female processors had no formal education compared to only 18.8% of their male counterpart. The evidence from the study concludes that at p =0.05, there are significance difference between gender participation and their ages (F=3.73, p=0.05), religion (F=4.167, p=0.044), household size (F=4.454, p=0.037) and sources of cassava (F=12.17, p=0.001). Also, significant difference exist between the attitude of male and female participating in cassava processing activities about the need for men’s strength (F=9.79, p=0.002), the availability of time on men’s part (F=5.01, p=0.03). However, no significant difference exists between male and female participation based on constraints faced with different processing techniques they are using. Finally, it is recommended that there is the need to motivate male participation in cassava processing activities, and that processing of agricultural products should not be seen as female job alone.