Increases in petroleum prices have affected the prices of its derived products. In addition, the interest in issues related to the environment and energy security at world-wide level has been increased. All of these factors have contributed to the development of alternative fuel such as the ethanol, biodiesel and natural gas. This study analyzes the main agricultural products used to produce biodiesel, their production costs and the utilization of those inputs in other industries. This study will also include a brief summary of different legislations in those countries under analysis. On the other hand, we will find break-even points and apply a sensibility analysis associated with distinct scenarios in terms of input-output prices.