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Abstract
The objective was to estimate the lethal and median reductive doses with gamma radiation, in seeds of Clitoria ternatea var. Tehuana, to induce genetic variation. Ten (10) doses of radiation (100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy) were used, plus one control (without radiation). The experimental design was completely random with three repetitions. The germination was evaluated 12 days after sowing (das), while the survival, plant height, stem diameter, number of leaflets and leaf area, at 30 das. The data were analyzed through ANOVA and the means comparison with the Dunnett test. The LD50 and GR50 for plant survival and height were estimated through linear regression. The radiation did not influence the germination and stem diameter; however, the survival was reduced significantly starting at 300 Gy, compared with the control (39.16%). Meanwhile, for plant height, leaflet number and leaf area it was from 100, 200 and 400 Gy (15.24, 22.54 and 70.26 %). The LD50 and GR50 was estimated at 350.91 and 306.1 Gy. The dose of gamma radiation to induce genetic variation in Clitoria ternatea var. Tehuana is suggested at between 306 and 350 Gy.