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Abstract
Sugar cane (Saccharum spp.) cultivation is affected by diseases that cause negative effects in its production, among which leaf scalding caused by the bacteria Xanthomonas alvilineans stands out. Multiple strategies have been used to decrease the damages caused by this disease, and the use of resistant varieties has been the most effective method for control; however, there is no reliable method for inoculation to evaluate the varieties in the disease. The inoculation methods, mechanical, injection and decapitation, were evaluated in five varieties with a known response to the disease; the causal agent was isolated and its molecular identification was performed. The results showed the causal agent with 99 % of identity and that the inoculation method by decapitation showed an incidence of 6.87 % and severity of 4.53 %, with significant differences compared to the two other methods.