[Objectives] To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis. [Methods] Based on the sucC gene of V. alginolyticus strain HY9901, specific primers were designed to amplify the full length sequence by PCR and make further analysis. [Results] The theoretical molecular weight of SucC protein was about 41 528.45 Da, and the full length was 1 167 bp, encoding 388 amino acids. It has no signal peptide and transmembrane region, and has a variety of functional sites. It is predicted that it is mainly located in the cytoplasm, and the ubiquitin and lactate modification sites overlap, and it has high gene homology with Vibrio parahaemolyticus. The α-helix, random coil and extended strand are the main secondary structures. The similarity between the constructed three-level structure model and the template is high. [Conclusions] This study reveals the structural characteristics and functional potential of SucC protein, and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.