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Abstract
During thawing and freezing of the sperm cell, the DNA structure may be damaged at different levels due to various internal and external reasons. In this study, DNA damage after freezing and thawing of functional masculinized rainbow trout (Oncorhynchus mykiss) semen was examined. Freezing was carried out using different dilution rates (1/3, 1/6, 1/9) and different doses of 1mM, 2 mM and 4 mM Ascorbic acid (antioxidant). In order to detect these damages that may be seen in the sperm DNA after the thawing process, the Comet assay method was used. The results showed that the group using 1/9 dilution ratio and 4 mM antioxidant dose gave the best results in terms of DNA damage. In this study, we tried to determine possible DNA damage after cryopreservation of functional rainbow trout sperm.