Objective: To quantify the one-year total soluble protein (TSP) concentration in the trunk of two pine species. Design/Methodology/Approach: The Bradford method (1976) was used to determine the TSP concentration in the two pine species. Statistical tests were subsequently performed with the IBM SPSS 18 Software, using a general linear model (GLM) univariate analysis. Results: The TSP concentration was different for each month. The highest concentration was recorded in August (6.84 mg gMS1 for Pinus pinceana Gordon and 6.82 mg gMS1 for Pinus cembroides Zucc), and the lowest was registered in April (5.53 mg gMS1 for Pinus cembroides Zucc) and February (6.64 mg gMS1 for Pinus pincenana Gordon). Study Limitations/implications: There is a lack of information regarding these two pine species and scarce studies explain the behavior of the TSP. Conclusions: The concentration of total soluble proteins varies in each month of the year. Further studies that include more plant organs are required to obtain a broader protein profile for both species.