Based on the analysis of the importance of professional cluster construction by ecological theory, with the change of social demand for talents, this paper explores the practice of environmental chemical professional cluster construction in Pingdingshan University, including gradually perfecting teaching conditions and reforming teaching mode, breaking through the limitations of resources, integrating the boundaries of colleges and departments, integrating multiple resources, innovating systems and mechanisms, reconstructing professional clusters, deconstructing professional connotations, reorganizing curriculum systems, etc., in order to better build the ecological chain network of education in application-oriented colleges and universities, realize the deep integration of industry and education, train future-oriented interdisciplinary applied talents of new engineering, and realize the construction of characteristic professional cluster in application-oriented colleges.