Starting from the transformation and upgrading of traditional culture, the optimization and development of traditional industries, and the innovation of traditional models, this paper used cultural entrepreneurship to trigger the thematic business engine, and explored Zhucheng City with its unique dinosaur culture. It proposed "Three-integration" rolling development mode to build Zhucheng into a new highland of cultural entrepreneurship and create new competitiveness under the background of agricultural and rural modernization development. This paper also explored from the perspective of multi-subject collaboration of cultural entrepreneurship. Government needs to establish rolling development pilot projects and promote their application; enterprises need to play the engine role of thematic business through the mode of "getting larger to help smaller ones"; the masses need to pay attention to the reaction of culture to the economy, so as to promote the wave of high-level promotion of cultural inheritance and urban-rural integration.