Huaiyin Institute of Technology is taken as the research object. The content of horticulture curriculum system under the background of new agricultural science is constructed and elaborated, including theoretical curriculum system of "three platforms and seven curriculum groups" and practice system of "two platforms, three levels and four modules". Moreover, the quality assurance strategy of the curriculum system is discussed from three angles of enhancing professional emotion, enhancing professional practice and strengthening innovation ability, to reach the training objective of outstanding applied horticultural talents under the background of new agricultural science.