[Objectives] To accurately determine the naringin content in Rhizoma Drynariae [Methods] The high performance liquid chromatography (HPLC) method was applied in the determination of the naringin content in Rhizoma Drynariae .The sample was sonicated at room temperature .The mobile phase was 0.1% phosphoric acid-acetonitrile (75:25), detected by diode array detector at the wavelength of 284 nm, and quantified by external standard method. [Results] The linearity of naringin was good in the concentration range of 5-500 μg/mL with a correlation coefficient of 0.999 9. [Conclusions] This method has good linearity, easy operation, correctness and reproducibility as required, and is expected to provide a method for the determination of naringin content in Rhizoma Drynariae