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Abstract

[Objectives] The paper was to analyze and predict the structure and characteristics of xylanase xynMF13-GH10 gene and its encoded protein. [Methods] xynMF13-GH10 gene was predicted by NCBI and various information analysis tools in ExPASy website, as well as SignaIP 5.0, DNAman, TMHMM, SOPMA and SWISS-Model, and the characteristics and functions of protein structure encoded by the gene were predicted. [Results] The gene is 1 332 bp in length, the coding region is 1-1 332 bp, and the gene encodes 443 amino acids.The xynMF13-GH10 gene has high homology with xylanase in many species, and it has the highest homology with Paraphaeosphaeria sporulosa endoxylanase-like protein, with the consistency reaching 78.91% and e-value reaching 2e-159. The secondary structure consists of 48.31% random curl, 30.25% α-helix structure, 16.70% extended chain and 4.74% β-corner. [Conclusion] The results provide a reference for revealing the physiological function and expression regulation mechanism of xynMF13-GH10 gene in the future.

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