[Objectives] To establish a breeding system for pre-elite seed potatoes. [Methods] Shoot-tip seedlings were cultured with different medium, and then transplanted in different substrates. They were sprayed with nutrient solutions of different formulas. Potato minitubers were induced. [Results] The medium of MS+2.0 mg/L 6-BA, 1.0 mg/L NAA+3% sugar was preferable for potato shoot tip culture. After virus detection, the virus-free potato seedlings were propagated. The medium composed of vermiculite, peat and perlite (1:3:1) was recommended for transplanting potato tissue culture seedlings in greenhouse. The three-dimensional spray method was used to produce pre-elite seed potatoes. The full-amount MS nutrient solution could increase the tuber number per plant to 55. Induction of minitubers is a better breeding technology for pre-elite seed potatoes. The optimal medium screened for induction of potato minitubers was MS + (5-7) mg/L 6-BA, with a dark culture duration of about 20 d. [Conclusions] The breeding technology established is suitable for the two-season cultivation areas in ​​Zaozhuang.