Experimental teaching is an important part of biology teaching in middle school. In the form of questionnaire, this paper conducts a survey on some leading teachers of biology in the middle schools in the Yangjiang area about the biological experiment teaching in the implementation of the new curriculum, and analyzes the results of the survey. The problems and deficiencies in the implementation of biological experiment teaching in some middle schools in this area are found, and the relevant reform measures and suggestions are put forward in order to promote the better development of biological experiment teaching in middle schools.