Town of Banská Bystrica, the middle-size regional centre (less than 80 thousand inhabitants) from central Slovakia has an eccentric geographical position in relation to the rest of the rural self-governing region of Banská Bystrica. This reality has an important impact on the public transport management from the perspective of the region and individual providers. The aim of the study is to identify municipalities and areas of this region which inhabitants are marginalised or excluded from using the public transport services to/from their regional centre. We used the concept of “daily accessibility” together with a priori accepted condition from the perspective of potential travellers about “the longest stay time in regional centre” as the decisive criteria for delimitation of territories (transport-disadvantaged areas), which inhabitants suffer not only from public transport exclusion, but also from the greater number of transport vehicle changes and used public transport lines (connections) during the return journey. Electronic database of train and bus timetables was used by procedure for obtaining the information about studied characteristics for return journey during 24 hours of working days (Wednesday) and the rest of the week (Sunday).