The breeding of replacements is fundamental in any production system, since the heifers are the one that will replace in a certain time the cows that gradually leave the exploitation. Probiotics can form part of the composition of different types of products, including foods (functional foods), medicines and dietary supplements. The objective of this study was to evaluate the feeding cost of Holstein heifers fed with whole milk added with Bacillus subtilis PB6. 60 new-born animals were used, randomly included in 1 of 3 treatments. The treatments were as follows: T1 = control, T2 = 10 g heifer day. The First Take within 20 min after birth, T3 = 10 g heifer day. The first takes between 12 and 24 h after birth. In All treatments 432 L of pasteurized whole milk was supplied divided into two outlets day 07:00 and 15:00 respectively, for 60 days, the addition of Bacillus subtilis PB6 was carried out in the milk tub at the time of feeding them. The first intake of colostrum (2 L•intake) per animal, was supplied within 2 h after birth, then provided a second 6 h after the first. The variables to evaluate the cost of feeding were considered milk consumption and concentrate during the first 60 days of life. It Was concluded that in the evaluated variables no statistical difference was observed P<0.05. In relation to the cost of feeding and the cost integrated by kilograms, it is observed lower cost for the T2, corresponding to the heading of the feeding of heifers with whole milk added with Bacillus subtilis PB6, improved the relation benefit-cost.