Horticultural Plant Biotechnology is a core course of undergraduate teaching of horticulture with high practicality. For students, its contents are too complex and abstract to master. In order to strengthen the teaching effect of the course, stimulate students' interest in learning, improve students' ability to analyze and solve problems, this article makes an in-depth analysis of the complicated and abstract knowledge points, the endless emergence of new knowledge, the disconnection between practice and experimental teaching in the Horticultural Plant Biotechnology course. Based on the analysis, it puts forward the reasonable and blend methods of online teaching, to cultivate innovative and practical ability of students.