Open data are important for adding legitimacy and transparency to public sciences. These data have also a potential to be used as a first approach for scientific investigation, such as spatial evaluation of ecosystem services. This paper presents a methodological approach to evaluate the trade-offs between agriculture and supporting ecosystem services based on spatial analysis and open data. The study area is an important agricultural production region in Bahia State, Brazil. The framework was able to establish the spatial interactions between agriculture and ecosystem service provision, while the regional scale was useful in supporting guidelines regarding sustainable land use for agricultural areas.