The contents of chlorogenic acid and luteoloside in Flos Lonicerae from Binhai New Area and Jinnan District of Tianjin were determined to provide basis for the quality identification of this medicinal material. The content of chlorogenic acid was determined by HPLC. In Flos Lonicerae from Binhai New Area and in Flos Lonicerae harvested at different stages from Jinnan District, the contents of chlorogenic acid were 3.804%, 5.507% (three green stage), 4.855% (silver flower stage) and 4.220% (golden flower stage), respectively, and the contents of luteoloside were 5.53%, 12.405% (three green stage), 14.370% (silver flower stage) and 0.917% (golden flower stage), respectively. The contents of chlorogenic acid in Flos Lonicerae from Jinnan District were higher than that from Binhai New Area. Among different stages, the content of chlorogenic acid was highest in three green stage, followed by that in silver flower stage. As the flowers bloomed, the content of chlorogenic acid in the medicinal material showed a significant downward trend. In Flos Lonicerae from Jinnan District, the content of luteoloside was highest in silver flower stage and lowest in golden flower stage.