The industrial prosperity proposed in the overall requirement of rural revitalization strategy in the Report of the 19th National Congress is the cornerstone of rural development. E-commerce poverty alleviation is an effective way of poverty alleviation in recent years. This paper mainly explored the factors influencing the rural e-commerce poverty alleviation and the evaluation of poverty alleviation effects, in order to come up with countermeasures and recommendations to provide assistance and guidance for the use of e-commerce to achieve poverty alleviation and accordingly to revitalize the rural industry. Firstly, using the literature research method, it proposed four level I indicators influencing the e-commerce poverty alleviation, and designed a detailed level II indicator system under each dimension. Then, the questionnaire survey method was employed to collect data for SPSS analysis to verify whether the designed indicator system is correct. Finally, it analyzed the problem of the current stage of e-commerce poverty alleviation by means of the quadrifid graph, and came up with corresponding countermeasures on the basis of the four key levek I factors.