[Objectives] This study aimed to investigate the inhibition effects of two chemical constituents in Salvia deserta Schang on human aldose reductase (AR). [Methods] The isolated salvianolic acid M and rosmarinic acid were prepared into different concentrations of solutions for in vitro enzyme kinetics experiments. [Results] Salvianolic acid M and rosmarinic acid had the strongest inhibition effects at the concentration of 1 g/L. [Conclusions] Salvianolic acid M and rosmarinic acid have inhibition effects on human aldose reductase. The IC50 of salvianolic acid M is 0.79 μg/mL, and that of rosmarinic acid is 1.13 μg/mL.