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[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves. [Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and the optimal extraction technology was obtained by box-benhnken test design analysis. [Results] After analyzing the results, the optimal process was as follows: a time of 28.89 min and liquid/material ratio of 31.38∶1, the temperature was 40.74 ℃. In this way, NaNO2-Al (NO3)3-NaOH was used as color developing agent, and the theoretical extraction of flavone in ginkgo leaf can reach 11.74 mg/g. [Conclusions] This method can be used to extract total flavonoids from G. biloba leaves with simple operation, low cost and good repeatability.


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