In this article, microbial fuel cell (MFC) was used for simultaneous denitrification and carbon removal to ascertain their electricity generation performance. The results showed that strengthening domestication and enrichment of electrogenic bacteria had the best start-up effect. An increase in volumetric loading reduced the rate of pollutant removal but promoted the output voltage. The changes of working conditions such as influent concentration, sludge concentration and temperature had a great influence on the electricity generation performance of MFC, and their optimum values were 500 mg/L, 2 000 mg/L and 35℃, respectively.