The research was conducted during 2013-2015 with two cotton cultivars - Helius and Darmy (Gossypium hirsutum L.). It was investigated five herbicides: Goal 2 E (oxyfluorfen) - 800 ml/ha; Linuron 45 SC (linuron) – 2 l/ha; Wing-P (pendimethalin + dimethenamid) – 4 l/ha; Merlin 750 WG (isoxaflutol) - 50 g/ha; Bazagran 480 SL (bentazone) – 1.5 l/ha. They were treated separated or combined with growth regulator Amalgerol 5 l/ha or foliar fertilizer Lactofol O – 8 l/ha in the budding stage of the cotton. It has been found that investigated herbicides and their combinations with growth regulator and foliar fertilizer do not have a phytotoxic effect on the fat content of the cotton seed cultivar Helius. These herbicides and tank mixtures reduce the fat content in the seeds of cultivar Darmy. The decrease is greatest in the Linuron 45 SC herbicide and its combination with Amalgerol. For the first time, it has been established that, from the point of view of the technology for cotton growing of cultivar Helius, technologically the most valuable are all herbicides and their combinations with growth regulator and foliar fertilizer. Technologically the most valuable in cotton cultivar Darmy are tank mixtures of herbicides Wing-P and Bazagran 480 SL with growth regulator Amalgerol. They combine high values and high stability of fat content in cotton seed during different years. The alone use of herbicide Linuron 45 CK and its combination with Amalgerol receives negative assessments and should be avoided. The most economically effective are tank mixtures of herbicides Wing-P and Bazagran 480 SL with growth regulator Amalgerol.