In order to provide scientific basis for rational fertilization of maize under different soil quality and fertility, the effect of combined application of NPK on yield and fertilizer effect of different maize varieties was studied by using "3414" experimental design scheme in this paper. The results showed that there were significant differences in ear length, ear diameter, barren ear tip length, kernel number per spike, kernel weight and yield among different fertilization treatments. Fertilization can improve the agronomic characters of maize and increase the yield. The yield increase of maize in two experimental plots (MHQ and HZY) was 11.0%—64.3% and 0.2%—61.9%, respectively. There was obvious interaction effect among nitrogen, phosphorus and potassium fertilizers, the yield of maize was the highest at the level of medium nitrogen, medium phosphorus and medium potassium fertilization, and the fertilizer effect was in the order of N＞P2O5＞K2O. The results of curve fitting showed that the recommended application rates of N, P2O5 and K2O in MHQ plot were 373.7，74.8 and 79.9 kg/ha, respectively; the recommended application rates of N, P2O5 and K2O in HZY plot were 419.7 , 75 and 75 kg/ha, respectively.