This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography (HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition, the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin, Cr, Cu, As, Cd and Pb in S. baicalensis planted in Jizhou District were 14.625 05%, 11.360 12.830 , 0.062 315 , 0.025 715 and 0.176 65 μg/g, respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard (≥ 9.0%) of Chinese Pharmacopoeia of 2015 edition, and the contents of heavy metals were also below the limit (≤20/1 000 000) required by Chinese Pharmacopoeia of 2015 edition.