In order to verify the accuracy of ion chromatography for cyanide detection, optimization conditions were studied, and comparison was made for the detection results of cyanide between titration and isonicotinic acid pyrazolone spectrophotometry. The results showed that ion chromatography has good linearity and reproducibility, with the recovery rate of 95% -105% and the limit of detection of 0.001 mg/L. This method is simple, rapid, safe, selective, and suitable for the determination of cyanide in pollution-free livestock product breeding water.