The effect of different ratio of organic and inorganic fertilizers on the growth and the yield and quality of flue-cured tobacco was studied. Using randomized blocks design, the data of the test materials K326 were analyzed by using variance analysis, significance test and other methods. The release efficiency of organic fertilizer in the soil was slower, and the effect of organic fertilizer on the growth of tobacco plants was worse than that of chemical fertilizers, and under 25% organic fertilizer+75% chemical fertilizer treatment, the quality of the flue-cured tobacco was good. Under the treatment of 50% organic fertilizer+50% chemical fertilizer, the production of flue-cured tobacco was high and the ratio of first-class tobacco was high. It is suggested that the organic fertilizer application ratio should be 25%-50% in the agricultural production.