Organizations have become more global, complex and competitive. The objective of the present study is to examine the dominant work culture prevalent in the two organizations X and Y and to study its impact on the motivation level of the employees at the managerial level so that a model can be developed. This research paper seeks to investigate the relationship between the various independent and dependent vriables through Correlation Analysis. Stepwise multiple regression analysis was undertaken to assess the significant predictors of work culture for the total sample. The findings show that Technocratic culture has the strongest partial correlation or the purest relationship with role motivation. Through regression analysis, technocratic culture emerges as a strong predictor of motivation.