Boron, one of the essential trace elements for normal growth and development of tobacco, has a great impact on the yield and quality of flue-cured tobacco. The objective of this study is to explore the best pattern and level of boron fertilizer supply of red soil in the Honghe tobacco area. In this study, the randomized block design was used to study different boron fertilizer supply methods and levels. The results showed that during transplanting, it was good to apply 1.50 kg/ha Fertibor boron with pure boron content≥15%; at the resettling stage, it was good to spray 225 kg solution composed of 75 mL/ha Compo liquid boron with pure boron content≥130g/L mixed with water onto leaves when night fell in a sunny day. The two fertilization methods had better effect than the basal application of boron fertilizer, and the resistance to pests and diseases or economic character of tobacco plants was better than under spraying treatment.