In recent years, farmers in Jamaica have experienced difficulties in obtaining seed potatoes in time, which resulted in lose of yields and production. At the same time, there is a growing interest in the introduction of new varieties. Tissue culture techniques for rapid propagation and for the introduction of new varieties as well as germplasm conservation could provide answers for some of these problems. Twenty-one varieties which include the locally grown commercial ones like Desiree, Spunta, Kenebec and some promising new varieties like L.T. 5, 6, 7, 8, 8, 9 and Atlantic, etc. have been subjected to various techniques including single node cuttings, multinode cuttings, liquid media, solid media, liquid cultures without shaking, in vitro tuber production and culture at low temperature. Plantlets obtained through tissue culture have been successfully introduced into the field with good yields of seed tubers and mother plants for vegetative propagation.