Subject and purpose of work: The subject of the work were business environment institutions as entities that affect the investment attractiveness of the region. The aim of the article was to identify and assess the role of business environment institutions in shaping the investment attractiveness of regions, with particular focus on park institutions and Regional Investor Service Centres. Materials and methods: The article highlights the significance of institutional factors in shaping the investment attractiveness, in the light of both the national and foreign literature. In addition, a presentation was made of the results of the qualitative research involving an assessment of the content posted on the websites of selected institutions that provide support for entrepreneurs in the provinces. Results and conclusions: Business environment institutions constitute an important factor for potential investment attractiveness of the region through their impact on the possibilities of acquiring, storing, transforming and sharing of knowledge between different entities. Institutional coverage of the regions also affects the ability to create cooperation networks, including the formation of cluster initiatives. It has also been proven that there is a dependency between institutional resources of the region and its actual attractiveness.