The detection and identification of economically important plant pathogens is of paramount importance in Florida and the Caribbean region. Invasive pathogens are particularly grave threats, as exemplified by the recent establishment of citrus canker, soybean rust, citrus greening, texas phoenix palm decline, and laurel wilt diseases in Florida. Accurate and rapid identification of such threats is fundamental to protecting agriculture in Florida, the United States, and Puerto Rico. This research is designed to increase the diagnostic capacity to reduce and possibly eliminate the harmful effects of such invasive species as listed above that have invaded the region or are a future threat to the region, and adversely affect production agriculture and the natural environment. Plant diagnostics play a crucial role in agriculture and ultimately impact society as a whole. Plant diagnosticians face numerous challenging situations, most of which have a major economic impact, such as diagnosing a non-endemic potentially invasive pathogen at a port of entry. In some instances, there may be life-threatening situations like identifying a poisonous plant or mushroom that was eaten by human or animal. Whatever the case may be, possessing the capability to provide clientele with accurate and reliable diagnoses should be a high priority for all diagnostic laboratories. Therefore, we present a novel PCR technique that, when used correctly, can detect and amplify plant pathogenic DNA directly from host tissue that is several orders of magnitude more sensitive than the Standard PCR. The potential use of the High-Fidelity PCR in routine plant diagnostics is readily obtainable, requiring the same equipment and expense used for the Standard PCR. This new technology has the potential to be developed into a diagnostic tool with unprecedented sensitivity and power to protect agriculture