Milk production belongs to conventional branches of agricultural production in the Czech Republic. Its results affect economics results of the whole animal production by significant way because milk sector participates by more than one third on the value of the animal production and almost by 17% on the rough agricultural production. The EU enlargement brought in this sector the amendment in the direction and volume of distribution flows both of raw material - i.e. raw cows´ milk and of processed milk products. The identification of amendments in the whole chain of production, processing and final utilization, including impacts on price development and estimation of future development should contribute to better understanding and adaptation of Czech farmers and Czech milk processors to new economic and business conditions.