With the forms of pistil stigma of "Guiti 2" Chinese water chestnut cultivar during the pollination as the object of observation, we reveal the pollination process of Chinese water chestnut from the microscopic point of view, to provide reference for Chinese water chestnut crossbreeding. The results show that the Chinese water chestnut pistil has 2-4 stigmas which present white filament and vascular bundle forms, and the vessels on epidermis are thick with long translucent branched hairs; after pollination, pollen grains are tightly bound on branched hairs, and after identification, the pollen tube can penetrate branched hairs, continue to grow, and transfer the genetic material in pollen to vascular bundle.