This study was carried out to determine the effects of age at prelay (15 and 19 weeks) and dietary supplementation of graded levels of Alphamune G (0.00, 0.04, 0.05, 0.06%) on laying performance of pullet chickens. The experiment period was 17 weeks and completely randomized design was employed. Feed intake, nutrient retention, weight gain and feed to gain ratio values were similar (p > 0.05) among birds fed the different dietary inclusion levels of Alphamune G. Hen day production, Haugh unit and Albumen height were significantly high (p < 0.05) for laying hens of fed the control diet. There was also interaction effect of Alphamune G and Age. The interaction of Alphamune G and Age gave the highest value in laying hens of group B fed the 0.05% diet when compared to the control. However, birds fed the 0.06% Alphamune G inclusion level gave the best result in terms of Production characteristics, Cost to benefit ratio expressed as Cost of feed to produce a Dozen Egg and Egg Quality. Also birds of group B gave better results than that of group A except in the Haugh unit value.